《植物生理学报》 2018, 54(9): 1475-1480

长穗偃麦草幼穗离体培养高频再生体系的建立

周妍彤^1,2, 张琳², 郭强², 田小霞², 孟林^2,*, 崔国文^1,*

¹东北农业大学动物科技学院, 哈尔滨150030; ²北京市农林科学院北京草业与环境研究发展中心, 北京100097

通信作者：孟林；E-mail: menglin9599@sina.com, cgw603@163.com

摘　要：

以长穗偃麦草(Elytrigia elongata)幼穗为外植体, MS为基本培养基, 研究了不同植物生长调节物质配比对其愈伤组织诱导、分化和生根的影响。结果表明, 长穗偃麦草幼穗诱导最佳培养基为MC+3 mg·L^-1 2,4-D, 诱导率达66.67%, 4周后可见淡黄色愈伤组织。最佳分化培养基为MC+0.1 mg·L^-1 2,4-D+3 mg·L^-1 6-BA, 分化率为64.44%, 4周后出现芽点, 同时伴随根毛发生。最佳生根培养基为MR+0.5 mg·L^-1 NAA, 生根率为100%, 移栽后全部成活｡

关键词：长穗偃麦草; 幼穗; 组织培养; 植株再生

收稿：2018-03-12   修定：2018-09-09

资助：北京市自然科学基金(6182013)和北京市农林科学院科技创新能力建设专项(KJCX20170110)。

Establishment of high frequency plant regeneration system from panicle in vitro culture of Elytrigia elongata

ZHOU Yan-Tong^1,2, ZHANG Lin², GUO Qiang², TIAN Xiao-Xia², MENG Lin^2,*, CUI Guo-Wen^1,*

¹College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China; ²Beijing Research and Development Center for Grass and Environment, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China

Corresponding author: MENG Lin; E-mail: menglin9599@sina.com, cgw603@163.com

Abstract:

The tissue culture and rapid propagation system of Elytrigia elongata was established by using the panicle as explants and MS as the basic callus. The effects of various plant grouth regulators combinations on callus induction, differentiation and rooting were studied. The results showed that the optimal medium for induction of panicles of E. elongata was MC+3 mg·L^-1 2,4-D, and the induction rate was 66.67%. After 4 weeks, light yellow callus was observed. The optimal differentiation medium was MC+0.1 mg·L^-1 2,4-D+3 mg·L^-1 6-BA, and the differentiation rate was 64.44%. After 4 weeks, the bud point appeared, accompanied by root hair. The best rooting medium was MR+0.5 mg·L^-1 NAA, the rooting rate was 100%, and all survived after transplanting.

Key words: Elytrigia elongata; panicle; tissue culture; plant regeneration